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. 2021 Aug 31;118(36):e2113288118. doi: 10.1073/pnas.2113288118

Fig. 3.

Fig. 3.

Caspase-8, caspase-3, and TRAF-7 overexpression increases sensitivity to TRAIL in cells with acquired resistance. (A) Down-regulation of caspase-8, caspase-3, FoxO3a, and TRAF-7 in TRAIL-resistant cells. The bands were quantified by ImageJ software and the relative values normalized by the value of each GAPDH. (B) qRT-PCR showing caspase-3 and caspase-8 mRNAs down-regulation in H460R compared with H460S cells. (C–E) Proliferation (C), caspase-3/7 assay (D), and Western blot showing PARP-1 cleavage (E) after caspase-3, caspase-8, and TRAF-7 overexpression in TRAIL-resistant cells. Error bars indicate mean ± SD (n = 3) and the P values were calculated by two-tailed Student t test (*P < 0.02, **P < 0.01).