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. 2021 Sep 2;118(36):e2100657118. doi: 10.1073/pnas.2100657118

Table 1.

Kinetic parameters for DcpG ligand-dependent DGC activity at 25 °C (mean ± SD of at least three independent experiments)

Ligation state kcat (min−1) KM (µM) kcat/KM (M−1/min−1)
Fe(II) 1.21 ± 0.07 284.4 ± 123.3 4,243
Fe(II)-O2 0.45 ± 0.04 80.9 ± 33.5 5,562
Fe(II)-NO 2.02 ± 0.36 443.7 ± 186.4 4,574
Fe(II)-CO 1.32 ± 0.09 236.6 ± 71.9 5,569
Fe(III) 0.22 ± 0.05 88.6 ± 35.0 2,483