Fig. 3.

PGC-1α chromatin recruitment is regulated by RNA binding at the CTD. (A) Volcano plot with green dots representing RNAs interacting with the CTD of PGC-1α. (B) Annotation of in vitro AP-seq peaks representing PGC-1α CTD bound RNAs. (C) Genome browser track view of representative peaks located at promoter (Top), distal intergenic (Middle), and intronic regions (Bottom). (D) Motif discovery analysis of PGC-1α CTD bound RNAs. (E) Overlap of transcripts that are differentially expressed (DEG), alternative-spliced, and bound by PGC-1α. (F and G) Protein content of PGC-1α FL bound to chromatin fractions in the absence and presence of 1 mg/mL of RNase A (F) or following the addition of 5 µg/µL of yeast tRNA (G). Immunoblots are representative of three independent experiments, each performed in triplicate.