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. 2021 Aug 30;118(36):e2105510118. doi: 10.1073/pnas.2105510118

Fig. 3.

Fig. 3.

The FERM domains SNX27 and SNX17 recognize the DLF and NPxY/NxxY motifs, respectively. (A) GST-SNX168-90, GST-SNX168-90DLF, GST-VLDLR, and GST pull-down of purified His6-SNX27-FERM. Shown is a Coomassie Blue–stained SDS/PAGE gel of purified protein (Left) and bound samples (Right). * indicates the retained His6-SNX27-FERM (Left) or MBP-SNX17-FERM (Right). (B) The GST-fusion proteins used in A were incubated with purified MBP-SNX17-FERM. Shown is a Coomassie Blue–stained SDS/PAGE gel of purified protein (Left) and bound samples (Right). (C and D) ITC of P-selectin, LRP1, SNX168-90, and SNX168-90 DLF titrated into MBP-SNX27-FERM (C) or SNX17 FL (D) in a buffer containing 50 mM Tris-Base pH 8.0 or 100 mM NaCl at 16 °C, respectively. Raw data are listed at the top, and integrated normalized data are listed at the bottom. Colors indicate the peptides used in the assay. Experiments were performed in triplicate, and one representative experiment is presented.