Fig. 2.

S1P(d18:1) levels are different between fractions in adult wild-type Atxn1[82Q]/−, and in Atxn1[82Q]/+ mice, S1P, ceramide, and sphingomyelin are increased in the anterior cerebellum. (A) Bar plot of HPLC-MS/MS quantification of selected sphingolipid levels in wild-type Atxn1[82Q]/− (FVB/N) and mutant Atxn1[82Q]/+ mice. (B) Schematic of the sphingolipid metabolic pathway with representation of level differences between anterior (red) and flocculonodular (pink) fractions. (C) Schematic of the sagittal view of the Atxn1[82Q]/+ cerebellum with identification of the fractions and annotation of the sphingolipid changes measured in the mutant in independent fractions. Data are expressed as mean $\pm$ SEM (n = 10 per group); the unit is picomoles per milligram of protein. Juvenile indicates P21 to P23, and adult indicates P143 to P153. Statistical analyses for LC-MS/MS lipid quantification were performed using Levene’s test for equality followed by Student’s t test paired to compare anterior vs. flocculonodular fractions (black asterisks) and unpaired to compare Atxn1[82Q]/− vs. Atxn1[82Q]/+ (purple asterisks), with Bonferroni correction for multiple comparisons. an., anterior; fn., flocculonodular; Cer/CER, ceramide; CERK, ceramide kinase; SM, sphingomyelin. *P < 0.05; **P < 0.005; ***P < 0.001.