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. 2021 Aug 31;118(36):e2107277118. doi: 10.1073/pnas.2107277118

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Fig. 2. — CAT-L713F fails to support ALC1-dependent nucleosome remodeling. (A) Scheme depicting a laterally positioned mononucleosome with a ³²P-label at the 5′ end and a linker DNA. The gray box indicates the position of a GAL4 binding site sequence in the linker DNA, and the asterisk indicates the radiolabeled end of the DNA fragment. (B) HhaI deprotection assay for nucleosome remodeling. (C) ATP and NAD⁺ dependence of ALC1 nucleosome remodeling activity. (D) Nucleosome remodeling assays included ALC1, PARP1, and/or CAT-L713F as indicated in the figure. ³²P-Nuc, ³²P-labeled nucleosomes; ND, not detected; t, time.