Figure 2.

Metabolic labeling of cPLA₂-expressing cells with [¹⁴C]ethanolamine. HEK293 cells were transfected with the insert-free vector (Mock) or the expression vector harboring cDNA for the indicated cPLA₂ isoforms tagged with FLAG. Their expressions were confirmed by Western blotting using anti-FLAG antibody (A). Arrowheads indicate the positions of the deduced molecular mass of each isoform. The cells were metabolically labelled with [¹⁴C]ethanolamine, followed by the treatment with ionomycin. Total lipids were then analyzed by TLC (B). The positions of the origin and authentic compounds are shown. The relative radioactivities of NAPE (C), NAE (D), and lysoNAPE (E) are shown (mean values ± S.D., n = 3). * p < 0.05.