Figure 3.

Metabolic labeling with [¹⁴C]ethanolamine of the cells co-expressing cPLA₂ε and one of the other isoforms. cPLA₂ε/Tet-on cells were transfected with the insert-free vector or the expression vector harboring cDNA for the indicated FLAG-tagged cPLA₂ isoforms or ABHD4 (AB4). Their expressions were confirmed by Western blotting using anti-FLAG antibody (A). Arrowheads indicate the positions of the deduced molecular mass of each isoform and ABHD4. The cells were labelled with [¹⁴C]ethanolamine, followed by the treatment with ionomycin (IM). Total lipids were then analyzed by TLC (B). The positions of the origin and authentic compounds are shown. The relative radioactivities of NAPE (C), NAE (D), and lysoNAPE (E) are shown (mean values ± S.D., n = 3). * p < 0.05.