Table 1.
Primers and mouse tissues used for the construction of expression vectors.
| cDNA (Accession Number) | Direction | Sequence (Restriction Sites and a Tag Sequence) | Tissue |
|---|---|---|---|
| cPLA2α (NM_008869) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataagtctttcatagatccttatcagcac-3′ (Spe I site, in-frame FLAG sequence) |
Thymus |
| reverse | 5′-cgcgcggccgcttacacagtgggtttacttagaaa-3′ (Not I site) | ||
| cPLA2β (NM_145378) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataaggctctgcaaacctgcccagtctac-3′ (Spe I site, in-frame FLAG sequence) |
Brain |
| reverse | 5′-cgcgcggccgctcactccggcctaaactgtttgcg-3′ (Not I site) | ||
| cPLA2γ (NM_001004762) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataaggaactaagctctggggtctgccct-3′ (Spe I site, in-frame FLAG sequence) |
Brain |
| reverse | 5′-cgcgcggccgcttaatccttagatatgttgtggga-3′ (Not I site) | ||
| cPLA2δ (NM_001024137) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataagtggagtggagatagaagagtaggc-3′ (Spe I site, in-frame FLAG sequence) |
Testis |
| reverse | 5′-cgcgcggccgctcacgtcttcactcccaatggcct-3′ (Not I site) | ||
| cPLA2ζ (NM_001024145) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataagccctggactctccagccaaagtgg-3′ (Spe I site, in-frame FLAG sequence) |
Large intestine |
| reverse | 5′-cgcgcggccgctcagcccccaacccttcccccagc-3′ (Not I site) | ||
| ABHD4 (NM_134076) |
forward | 5′-cgcactagtggaaaatggattacaaggatgacgacgataaggctgatgatctggagcagcagcctcag-3′ (Spe I site, in-frame FLAG sequence) | Brain |
| reverse | 5′-cgcgcggccgctcagtcaactgagttgcagatctcttc-3′ (Not I site) |