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. 2021 Aug 25;26(17):5152. doi: 10.3390/molecules26175152

Figure 6.

Figure 6

Effects of PTX and ASX microparticles on irradiated J774A.1 macrophages. (a) Limiting dilution assays were performed with non-irradiated cells to estimate parameter ε in Equation (1) (see the main text for details). Cells were seeded at each indicated density into the wells of 96-well culture plates and treated with ASX microparticles (P-ASX) and PTX, either alone or in combination (P-ASX + PTX), or left untreated (ctrl). F0 is the fraction of cells where no proliferation was observed at the end of the observation period (~30 days). When the cells are randomly and independently distributed, F0 is expected to obey Poisson statistics with parameter ε. Linear fitting of log-transformed data provided the following estimates: εctrl = 0.328 ± 0.0325, εP-ASX = 0.336 ± 0.036, εPTX = 0.351 ± 0.036, εP-ASX+PTX = 0.364 ± 0.038. The differences in the estimated parameter values were not statistically significant (ANOVA test, p = 0.9). (b) Survival probability of independent cell populations treated with ASX microparticles (P-ASX), PTX, P-ASX in combination with PTX (P-ASX + PTX) or left untreated (ctrl) and then irradiated with a dose of 4 Gy γ-rays. Nonlinear fits with Equation (1) allowed to estimate the fraction of the cells surviving radiation treatments (see also the main text for details). The results are: S(D)ctrl = 0.0071 ± 0.0005 (χ2/df = 1.5), S(D)P-ASX = 0.0086 ± 0.0006 (χ2/df = 1.2), S(D)PTX = 0.0095 ± 0.0007 (χ2/df = 1.7), S(D)P-ASX+PTX = 0.0131 ± 0.001 (χ2/df = 2.1). All observed differences in estimated parameter values were statistically significant as evaluated by ANOVA (p = 1.5 × 10−12) followed by Tukey post-hoc test.