Figure 1.

The cytotoxicity effects of bavachinin in NSCLC cells. (A) The chemical structure of bavachinin. (B) Three different NSCLC cells and 16HBE lung epithelial cells were seeded and incubated with bavachinin (0, 10, 20, 30 μM) for indicated time intervals. After incubation, cell viability was analyzed by MTT assay. (C) The NSCLC cells were seeded and stimulated with indicated concentrations of bavachinin (0, 10, 20, and 30 μM) for 24 h. Thereafter, bavachinin-containing media were replaced to complete culture medium every two days for 13 days. The culture was then washed, fixed, and stained with crystal violet. Colonies were counted as described in the Material and Methods. All results were at least triplicated and represented by the mean ± S.D. The statistically significant difference was labeled as * when p < 0.05 between control and experimental group.