Figure 5.

p38 MAPK-p21^Waf1/Cip1 signaling pathway involved in bavachinin-induced G2/M cell cycle arrest. A549 cells were pre-treated with or without 10 μM of SB203508 for 1 h before bavachinin incubation for 24 h. After treatment, (A) the protein expressions of p-p38 MAPK, p38 MAPK, p21^Waf1/Cip1, and β-actin, and (B) the mRNA expressions of p21^Waf1/Cip1, (C) the cell cycle distributions, and (D) the cell viability were determined. All results were triplicated and represented by the mean ± S.D. The statistically significant difference was labeled as * when p < 0.05 between the control and the experimental groups.