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. 2021 Sep 6;14(17):5104. doi: 10.3390/ma14175104

Figure 4.

Figure 4

GO-PEG-PEI/siRNA composite for facilitation of osteogenesis (A) schematic diagram of GO-PEG-PEI/siRNA composite. (B) Optical and fluorescence microscopy of MC3T3-E1 cells cultured on Wet polished Ti (PT), NT, and NT-GPP/siRNA substrate. (C) Proliferation assay (D) ALP activity (purple), collagen secretion (pink), and ECM mineralization (orange), and osteogenic gene expression of cells cultured on PT, NT, and NT-GPP/siRNA substrate. Each symbol represents significant difference between groups (** and *** p < 0.01 and 0.001 vs. PT. ## and ### p < 0.01 and 0.001 vs. NT. &&& p < 0.001 vs. NT–GPP. ^^^ p < 0.001 vs. NT–GPP/siNC). Data reproduced from Ref. [81]. Copyrights ACS 2017.