Figure 1.

HNE substrate selectivity (A) heat map representation of tetra-peptide-pNA sparse matrix library screened for activity in HNE kinetic assays. Bright green cells represent peptides that showed high rates of HNE proteolysis, black cells represent peptides that showed no detectable HNE proteolysis. Each square represents the average rate from three replicate experiments of triplicate measurements. Final assay concentrations were 300 µM peptide-pNA and 1 µg/mL HNE. (B) HNE consensus sequence derived from the MEROPS database showing amino acid frequency distribution between positions P4 and P4′. Amino acids are coloured according to sidechain chemistry: black = non-polar; green = polar; red = negatively charged and blue = positively charged.