FIG. 1.

Functional analysis of hTAF_II28 mutants. (A and B) Graphic representation of luciferase assays (arbitrary units). Cells were transfected with the vectors shown below each lane. Transfections contained 250 ng of the G4-ER or G4-VDR expression vectors, 1 μg of the TBP expression vector, 4 μg of the hTAF_II28 expression vectors, 5 μg of the luciferase reporter vector, and 2 μg of the pXJ-β-galactosidase reporter as an internal control. All transfections contained the appropriate ligands. The structures of the G4-NR activator plasmids and the luciferase reporter are schematized. The numbers represent the amino acid coordinates in the natural receptors. The mutants used in panels A and B are shown below the graph in panel A. (C) Transfections were as described for panels A and B. The mutants used are schematized below the graph. (D) Transfections contained 2 (lane 9) or 4 (lanes 5, 6, and 10) μg of the hTAF_II18 expression vector as indicated along with the amounts of the other expression vectors described above. WT, wild type.