FIG. 2.

Detection of bacterial DNA in clinical isolates by broad-range PCR. (A) Universal PCR with primers DG74 and 65ab. (B) Gram-positive-specific PCR with primers DG74 and 143. (C) Gram-negative-specific PCR with primers DG74 and 68d. PCR products were analyzed by 2% agarose gel electrophoresis and ethidium bromide staining. Lanes M, molecular size markers; lanes 1, Bacillus subtilis; lanes 2, Citrobacter species; lanes 3, Corynebacterium species; lanes 4, Enterobacter cloacae; lanes 5, Enterococcus species; lanes 6: E. coli; lanes 7, K. pneumoniae; lanes 8, Serratia liquefaciens; lanes 9, S. aureus; lanes 10, Streptococcus pneumoniae; lanes 11, negative control.