Fig. 3.

Cells in these cultures form synapses and exhibit electrophysiological activity. (A) Images of cultures 2 weeks post-differentiation (Ai) immunocytochemically labelled to determine synapsin expression and negative control image (Aii). Images were taken at 40 x magnification and scale bar represents 10 µm. (B&C) Representative whole-cell patch-clamp recordings from cells 2 weeks post differentiation showing (B) inward sodium currents in response to depolarising voltage steps and (C) repetitive firing of action potentials in response to current injection. Voltage-clamp and current-clamp protocols are depicted beneath raw data in (B) and (C).