Table 3.
Transitions table for peptides from apoA-1 used to study MPO-modified apoA-1. Each line corresponds to a native or an oxidized (Ox) peptide carrying a residue of interest. The entire sequence of the peptide with the residue of interest, the retention time (RT), the m/z of precursor ion and the two product ions are defined. The site of fragmentation is detailed for each product ion, they are usually simple (+) or double (++) positively charged.
| Peptide Sequence | Modification | RT (min) | Precursor Ion m/z | Product Ion 1 | Product Ion 2 | ||
|---|---|---|---|---|---|---|---|
| m/z | Frag | m/z | Frag | ||||
| 46LLDNWDSVTSTFSK59 | W50 | 10.7 | 806.90 | 199.18 | a2+ | 1271.59 | y11+ |
| 46LLDNWDSVTSTFSK59 | W50 Ox | 9.93 | 814.89 | 199.18 | 1287.59 | ||
| 62EQLGPVTQEFWDNLEK77 | W72 | 11 | 966.97 | 258.11 | b2+ | 838.42 | y14++ |
| 62EQLGPVTQEFWDNLEK77 | W72 Ox | 11.4 | 974.97 | 258.11 | 846.42 | ||
| 108WQEEMELYR117 | W108 M112 | 9.2 | 642.29 | 969.43 | y7+ | 315.15 | b2+ |
| 108WQEEMELYR117 | W108 M112 Ox | 8.12 | 650.29 | 985.43 | 315.15 | ||
| 108WQEEMELYR117 | W108 Ox M112 | 8.12 | 650.29 | 969.43 | 338.18 | y2+ | |
| 140LSPLGEEMR149 | M148 | 8.5 | 516.26 | 416.20 | y7++ | 621.26 | y5+ |
| 140LSPLGEEMR149 | M148 Ox | 7.37 | 524.26 | 424.20 | 573.26 | y5-64+ | |
| 216QGLLPVLESFK226 | Control 1 | 11.9 | 615.86 | 819.46 | y7+ | ||
| 216QGLLPVLESFK226 | Control 1 labelled | 12 | 619.36 | 826.48 | |||