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. 2021 Aug 31;13(17):2971. doi: 10.3390/polym13172971

Table 3.

Previous research on biological-chemical pretreatment approaches to pretreat lignocellulosic biomass. Data retrieved from Ref. [54].

Substrate Conditions Component’s Degradation (%)
1st Step 2nd Step Lignin Hemicellulose Cellulose
Biological—alkaline pretreatment
Corn stalks Irpex lacteus (28 °C, 15 d) 0.25 M NaOH solution
(75 °C, 2 h)
80 51.37 6.62
Populus tomentosa Trametes velutina D10149 (28 °C, 28 d) 70% (v/v) ethanol aqueous solution containing 1%(w/v) NaOH (75 °C, 3 h) 23.08 22.22 18.91
Willow sawdust Leiotrametes menziesii (27 °C, 30 d) 1% (w/v) NaOH (80 °C, 24 h) 59.8 68.1 51.2
Abortiporus biennis (27 °C, 30 d) 54.2 51.8 29.1
Biological—acid pretreatment
Populus tomentosa Trametes velutina D1014 (28 °C, 56 d) 1% sulphuric acid (140 °C, 1 h) 23.82 75.96 (+) 18.74
Oil palm empty fruit bunches Pleurotus floridanus LIPIMC996 (31 °C, 28 d) Ball milled at 29.6/s for 4 min. Phosphoric acid treatment (50 °C, 5 h) (+) 8.29 60.63 (+) 37.52
Olive tree biomass Irpex lacteus (Fr.238 617/93) (30 °C, 28 d) 2% w/v H2SO4 (130 °C, 1.5 h) (+) 105.82 75.29 (+) 62.95
Biological—oxidative pretreatment
Corn Straw Echinodontium taxodii (25 °C, 15 d) 0.0016% NaOH and 3% H2O2 (25 °C, 16 h) 52.00 23.64 (+) 45.45
Hemp chips Pleurotus eryngii (28 °C, 21 d) 3% NaOH and 3% (v/v) H2O2 (40 °C, 24 h) 55.7 23.2 25.1
Biological—organosolv pretreatment
Sugarcane straw Ceriporiopsis subvermispora (27 °C, 15 d) Acetosolv pulping (Acetic acid with 0.3% w/w HCl) (120 °C, 5 h 86.8 93.8 32.1
Pinus radiata Gloeophyllum trabeum (27 °C, 28 d) 60% ethanol in water solvent (200 °C, 1 h) 74.26 80.74 -
Biological—liquid hot water (LHW) pretreatment
Soybean Liquid Hot water (170 °C, 3 min, 400 rpm, 110 psi, solid to liquid ratio of 1:10) Ceriporiopsis subvermispora (28 °C, 18 d) 36.69 41.34 0.84
Corn stover 41.99 42.91 7.09
Wheat straw Hot water extraction (HWE) (85 °C, 10 min, solid to liquid ratio of 1:20) Ceriporiopsis subvermispora (28 °C, 18 d) 24.87 13.19 1.86
Corn stover 30.09 28.14 4.96
Soybean 0.09 0.09 0.09
Biological—steam explosion pretreatment
Beech woodmeal Phanerochaete chrysosporium (37 °C, 28 d) Steam explosion (215 °C, 6.5 min) 42.00 - -
Sawtooth oak, corn and bran Lentinula edodes (120 d) Steam explosion (214 °C, 5 min, 20 atm) 17.1 80.43 (+) 5.19

(+): represents the increment in fibre content.