Figure 5.

IL-2 Improves ILC2 Function During Acute MI in Rag2^−/− Mice

IL-2/Jes6-1 treatment enhances serum cytokine secretion of IL-5 at Day 3 post-MI (A). Intracellular detection of IL-5 (B, black histogram FM1, blue PBS, and red IL-2 treated) complements this. Splenic ILC2s (C) were expanded and expressed more IL-5 per cell. Splenic macrophages slew toward Arg1+ population (M2-like). PcAT ILC2s (C) were not expanded but expressed more IL-5 with biased macrophage population toward Arg1+ expression. Heart resident ILC2s (C) were not expanded but expressed more IL-5 per cell. Subsequent heart-infiltrating macrophages slew toward the Arg1+ population (M2-like). Mann-Whitney U test. Arg1+ = Arginase1+; PBS = phosphate-buffered saline; other abbreviations as in Figures 1 and 4.