Figure 1.

Inhibition of TGFβ2 using different KRAB-dCAS9 expression vectors and sgRNAs in GTM3 cells. GTM3 cells were transfected with pHR-SFFV-KRAB-dCAS9-P2A-mCherry (A), pLVX-EF1α-KRAB-dCAS9-P2A-mCherry (B), or pLVX-CMV-KRAB-dCAS9-P2A-mCherry (C) vectors (plasmids) together with the Lentiguide-Puro vector (plasmid) containing indicated sgRNA expression cassettes. Four days after transfection, whole cell lysates were used for Western immunoblotting. GAPDH was used as a loading control. Naive: TM cells that were not subject to any treatment. NT: nontargeting.