Figure 2.

Inhibition of TGFβ2 in primary HTM cells using KRAB-dCAS9 and sgRNA lentiviral expression vectors. Two primary HTM cells strains, HTM 071 (A) and HTM 2019-009 (B) were transduced with the pHR-SFFV-KRAB-dCAS9-P2A-mCherry lentiviral vector and the Lentiguide-Puro lentiviral vector containing indicated sgRNA expression cassettes. Four days after transduction, whole cell lysates were used for Western immunoblotting. GAPDH was used as a loading control. Naive: TM cells that were not subject to any treatment. NT: nontargeting.