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Binding of KRAB-dCAS9 to the promoter region of TGFβ2. GTM3 cells were transduced with pHR-SFFV-KRAB-dCAS9-P2A-mCherry lentiviral vector and the Lentiguide-Puro lentiviral vector containing indicated sgRNA expression cassettes. After ChIP assay using anti-dCAS9 antibody or IgG, purified DNA was used for qPCR using two primer sets (Primer 1 and 5) and fold of enrichment was calculated and shown. Three independent repeats were conducted.
