Figure 4.

CRISPR interference inhibited TGFβ2 expression in GTM3 cells. A transgenic GTM3 cell line (GTM3-ΔhTGFβ2^C226S/C228S-AcGFP; this line was established for the initial plan) that expresses ΔhTGFβ2^C226S/C228S-AcGFP (A) and some GTM3 cells that were transduced with the Ad5-CMV-ΔhTGFβ2^C226S/C228S viral vector (B) were further transduced with the pHR-SFFV-KRAB-dCAS9-P2A-mCherry lentiviral vector and the Lentiguide-Puro lentiviral vector containing indicated sgRNA expression cassettes. Four days post transfection, whole cell lysate was harvested for Western immunoblotting. GAPDH was used as a loading control.