Figure 2.

Protective effects of hOXR1 in the 661W cone-derived photoreceptor cell line after H₂O₂ treatment. (A) ROS levels in 661W cells overexpressing hOXR1 normalized to control cells was determined using DCF-DA. Fluorescence was measured 20 minutes after treatment with the indicated concentrations of H₂O₂. (B) Caspase activity of 661W cells overexpressing hOXR1 (MVL120 cells) normalized to control (CTL) cells was determined with the Caspase 3/7-Gloassay 16 hours following the addition of the indicated concentrations of H₂O₂. (C) Cell titer-Gloassay was used to determine percent cell viability 16 hours after the addition of the indicated concentrations of H₂O₂. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, ns = nonsignificant, 2-way ANOVA test. Data are mean  ±  SD from two independent experiments, with three sets of samples per experiment.