Effects of addition of ICZ at different time-points after HCMV infection. HFF cells were infected with HCMV AD169 at an MOI of 0.1 and were then treated with ICZ or GCV at 0, 3, 6, 9, 22, 26, 29, 33, 46, 50, 53, 57, 70, 74, 77, and 80 h p.i. Virus titers derived from HCMV-infected cells treated with the drugs at each time point were determined by titration on fresh HFF monolayers. Graph reports the mean ± SD of n = 3 independent experiments in duplicate.