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. 2021 Mar 20;31(8):1026–1036. doi: 10.1093/glycob/cwab018

Fig. 1.

Fig. 1

(A) DMBT1 and S8L expression in human nasal lavage aspirates from CRSwNP, CRS and control subjects. Representative immunoblot of nasal lavage samples from 12 patient subjects was double labeled for DMBT1 (left: IRDye 680RD donkey anti-rabbit IgG) and S8L (center: IRDye 800CW donkey anti-goat IgG) and signals overlaid (right). Arrow shows migration of molecular weight marker at 900,000 Daltons. (B) Sialidase sensitivity of S8L, but not DMBT1, in human nasal lavage from CRSwNP subject. Immunoblot was double labeled for DMBT1 (left) and S8L (center) and signals overlaid (right) in the absence (–) and presence (+) of V. cholera sialidase (100 mU/mL as described in Methods). Arrow = 900 kDa. (C) Induction of S8L expression in CRSwNP. Right panel shows staining intensity of bands from immunoblot analysis of nasal lavage aspirates compared from CRSwNP (n = 30), CRS (n = 9) and control (n = 16). *P < 0.05 CRSwNP vs. control, **P < 0.02 CRSwNP vs. CRS by Kruskall–Wallis test. Middle panel shows subgroup analysis after removal of subjects with oral steroid or immunomodulatory drugs from CRSwNP (n = 23), CRS (n = 9) and control (n = 15) subjects. *P < 0.05 CRSwNP vs. control, ***P < 0.03 CRSwNP vs. CRS by Kruskall–Wallis test. Left panel shows subgroup analysis of CRSwNP subjects specifically exposed to oral and/or inhaled steroids (n = 19), CRS (n = 9) and control (n = 16) subjects. *P < 0.05 CRSwNP vs. control, +P < 0.01 CRSwNP vs. CRS by Kruskall–Wallis test.