Figure 1.

Schematic representation of SDE. Basic SDE involves three key stages: gene diversification, selection and amplification. Initially, the target gene of interest can be employed for localized diversification using several molecular diversification tools. Later, the generated variant gene pool will be subjected to the iterative selection pressure to enrich the variants with superior functional qualities. In the final stage, the functional gene variants can be employed for further continuous gene diversification to amplify the superior functional characteristics.