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(a) Multicellular structures like spheroids and organoids can be embedded in a hydrogel-filled chamber of a microfluidics device. (b) A microfluidics device with TCR-engineered T cells (blue) and tumor spheroids (green) was imaged over time to assess the degree of T cell-induced cytotoxicity (red) (ref [151]). (c) 3-D tumor micropatterns cultured under a gradient of hypoxia were treated with CAR T cells to resolve spatial and temporal cytotoxicity patterns (ref [165]).
