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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Nat Biotechnol. 2021 May 6;39(9):1086–1094. doi: 10.1038/s41587-021-00910-x

Extended Data Fig. 8 ∣. XRBS profiling of limited human bone marrow cell types.

Extended Data Fig. 8 ∣

A) Plots show the gating strategy for fluorescence assisted cell sorting (FACS) of human bone marrow of CD34+ HSPCs, CD3+ T cells, and CD14+ monocytes. Singlets (FSC-W vs. -H) and viable cells (PI vs. FSC-A) were sorted based on cell surface marker signal.

B) Plot shows unique reads as a function of aligned reads in libraries from unsorted human bone marrow, HSPCs, monocytes, and T cells. Libraries were generated from 100 sorted cells. 1000 cells were used for the unsorted bone marrow library.

C) Heatmap depicts 4kb regions (rows, n=2,170 regions) centered over elements defined in the ENCODE SCREEN database. Only differentially methylated elements between monocytes and T cells are shown. Elements were stratified by their methylation status in HSPCs (hypomethylated: top; hypermethylated: bottom). Methylation levels of the unsorted bone marrow are shown for comparison (left). ATAC-seq signal for sorted hematopoietic stem cells (HSCs), monocyte, and CD4+ T cells (obtained from 36).