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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: Acta Biomater. 2021 Jun 18;132:473–488. doi: 10.1016/j.actbio.2021.06.025

Fig. 3. Scaffold morphology in the MCF7-S, P-hTMES and I-hTMES during on-chip culture.

Fig. 3.

Microscopy imaging of (a) MCF7-S; (b) P-hTMES; and (c, d) I-hTMES in Alg/Alg-S. Imaging conducted using epifluorescence microscopy (Zeiss Axio Observer.Z1) in ((a), two upper panels), (b), and (c), with MCF7 cells labeled with CFSE (green), fibroblasts with CMAC (blue), and dead cells with EtHD (red), using 20X magnification and 2 μm Z-stacking, Scale bar: 50 μm. Confocal imaging (Zeiss LSM 880) in (a), two lower panels, was conducted using 20X magnification, 2 μm Z-stacking, with MCF7 cells labeled with CFSE (green), tubulin labeled with anti-human tubulin mAb (red) and cell nuclei with Hoechst 33342 (blue), scale bar: 10 μm, upper panel, and 5 μm for magnified images (lower panel).Confocal imaging in (d) was conducted using 20X magnification, 2 μm Z-stacking, with MCF7 cells labeled with CFSE (green), macrophages with CMPTX (red), and cell nuclei with Hoechst 33342, Scale bar: 20 um, and 5 μm in the magnified image.