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. Author manuscript; available in PMC: 2021 Sep 12.
Published in final edited form as: J Phys Chem B. 2020 Apr 30;124(19):3892–3902. doi: 10.1021/acs.jpcb.0c01444

Figure 2.

Figure 2.

Functionalization of ultrasmall AuGSHzwt with Strep-tag. (a) Number of Strep-tag or Strep-tag(FITC) ligands attached per AuGSHzwt as a function of the peptide:AuMBA feed ratio (from 5:1 to 200:1). A maximum of ~ 10 Strep-tags are incorporated per AuNP at the highest feed ratio of 200:1. Quantification was performed by fluorescence spectroscopy using the emission signal from tryptophan or FITC. (b) Percentage of AuGSHzwt particles bound to Streptactin-coated beads as a function of the peptide:AuMBA feed ratio. The use of sufficiently low feed ratios (1:5 or 1:10) results in bound fractions smaller than 10% (dashed line), which implies monofunctionalization of the particles53.