Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Thermophoresis binding curves obtained for free Strep-tag titrated against free fluorescently-labeled Streptactin. Data obtained in (a) PBS, (b) 20 mg/mL BSA, (c) 50% FBS and (d) 50% FBS supplemented with a cocktail of protease inhibitors. Strep-tag was pre-incubated in the different media for either 1 h (squares) or 24 h (circles). Dashed lines mark the approximate values of apparent binding affinity.
