FIGURE 6.
Split‐ubiquitin yeast two‐hybrid assays for Actinidia virus D (AcVD) proteins. Proteins N, P, P3, G, and P6 were individually fused to the C‐terminal half of ubiquitin (Cub) of vector pDHB1, and proteins N, P, P3, and M were individually fused to the N‐terminal half of ubiquitin (NubG) of vector pPR3‐N. Yeast cotransformed with pDHB1‐largeT/pDSL‐P53 was used as a positive control, and yeast cotransformed with pDHB1‐largeT/pPR3‐N was used as a negative control. Yeast growth on synthetic dropout medium lacking leucine and tryptophan (SD−LW) was used to confirm the presence of both plasmids. Medium lacking leucine, tryptophan, histidine, and adenine (SD−LWHA) was used to screen for positive interactions. A series of dilutions (10−1, 10−2, and 10−3) is shown