FIGURE 5.

Increased NEDDylated proteins in cystic cholangiocytes, mostly involved in the translational machinery. (a) Representative immunoblotting of NEDD8‐IP proteins in normal and cystic human cholangiocytes (NHC and PHC, respectively). (b) Volcano plot of all identified NEDD8‐IP proteins (n = 140) by mass spectrometry comparing fold enrichment in PHCs to NHCs. Proteomic analyses of significant identified proteins (n = 27) in PHCs and NHCs by (c) heatmap representation, (d) gene ontology analysis, and (e) protein–protein interaction (PPI) network. Line color in PPI network indicates type of interaction evidence