FIGURE 1.

Caspase activation by different cell stressors in Casp9 ^−/− MEFs and effect of knockdown of caspase‐8 or ATG5. (A) Whole cell lysates from Casp9 ^+/+ and Casp9 ^−/− MEFs were immunoblotted for pro‐caspase‐9 and ACTIN. (B) Casp9 ^−/− MEFs were treated for the indicated times with brefeldin A (BFA), etoposide and paclitaxel or were γ‐irradiated and heat shocked followed by recovery for up to 72 h. Immunoblots for cleaved caspase‐8 and cleaved caspase‐3 are shown. Casp9 ^−/− MEFs stably transduced with (C) empty vector (EV) and Casp8 shRNA or with (D) EV and Atg5 shRNA were treated with brefeldin A (BFA), etoposide and paclitaxel for the indicated times or γ‐irradiated and heat shocked followed by recovery for up to 72 h. Immunoblots for (C) cleaved caspase‐8, pro‐caspase‐8, cleaved caspase‐3 or (D) ATG5‐ATG12, cleaved caspase‐8 and caspase‐3 are shown. ACTIN was used as a loading control