FIGURE 6.

The role of the stressosome in cell death of Casp9 ^−/− MEFs is dependent on the type of stress stimuli. Casp9 ^−/− MEFs stably transduced with scrambled shRNA and (A, C) Casp8 shRNA or (B, C) Atg5 shRNA were treated with BFA, etoposide, paclitaxel for the indicated times or exposed to γ‐irradiation or heat shock and allowed to recover for up to 72 h. (A) PI or (B) ToPro3 uptake was analysed at the indicated time points after treatment. (C) Following treatment for 72 h the culture medium was changed and cells were left to form colonies. Clonogenic survival assay was performed 10 days later