FIGURE 7.
Overexpression of cFLIPL leads to reduced stressosome‐mediated caspase activation. (A–C) Casp9 −/− MEFs were transfected with empty vector (EV) or cFLIPL. (A) Whole cell lysates were immunoblotted for FLAG‐tag and ACTIN. (B and C) Following transfection Casp9 −/− MEFs were treated with brefeldin A (BFA), etoposide and paclitaxel for the indicated times or exposed to γ‐irradiation or heat shock followed by incubation under standard conditions for up to 72 h. (B) Immunoblots show cleaved caspase‐8 and caspase‐3 and ACTIN. (C) Analysis of PI uptake was performed at the indicated time points after treatment