FIGURE 3.

FGFR1 promotes proliferation and inhibits apoptosis of osteoblasts. pcDNA3.1‐FGFR1 and siRNA‐FGFR1 were transfected into MC3T3‐E1 cells. A, EdU assays were used to assess cell proliferation. Scale bar=50 μm. B, CCK‐8 assays examined cell proliferation. C, PCNA, CDK4 and Cyclin D1 mRNA expressions. D, PCNA, CDK4 and Cyclin D1 protein expressions. E, Flow cytometry evaluated cell apoptosis. F, Cells were stained with Hoechst. Scale bar=50 μm. G, Bax, Bcl‐2 and cleaved caspase‐3 protein expressions. Data are shown as the mean ±SD. *P < 0.05, **P < 0.01