KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Bacterial and Virus Strains | ||
| AAV9-EF1a-FLEX-SNAPmGluR2-WPRE-hGH | Acosta-Ruiz et al. 2020 | N/A |
| AAV9-pCAG-FLEX-EGFP-WPRE | Oh et al. 2014 | Addgene Cat#51502-AAV9 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| DIPEA | Roth | Cat#4105.1 |
| DBU | Roth | Cat#6869.1 |
| DMF | Roth | Cat#5921.1 |
| DMSO | Roth | Cat#7029.1 |
| Formic acid | Merck | Cat#5.33002.0050 |
| TFA | Acros TCI |
Cat#139721000 Cat#T0431 |
| TSTU | Roth | Cat#2130.2 |
| HBTU | Roth | Cat#2132.3 |
| MeCN | VWR | Cat#83640.320 |
| (((9H-Fluoren-9-yl)methoxy)carbonyl-D-glutamic acid | ChemCruz | Cat#sc-485525 |
| Fmoc-Lys-Cy3 | Acosta-Ruiz et al. 2020 | N/A |
| Fmoc-Lys-Cy5 | Acosta-Ruiz et al. 2020 | N/A |
| THF | VWR | Cat#457071000 |
| XPhos Pd G2 | Aldrich | Cat#741825-1g |
| Cs2CO3 | Roth | Cat#6873.2 |
| 4-iodoaniline | Aldrich | Cat#129364-25g |
| nitrosobenzene | Aldrich | Cat#N24609-10g |
| acetyl chloride | Aldrich | Cat#114189-25g |
| NaH, 60% in mineral oil | Aldrich | Cat#452912-100g |
| methyl iodide | Aldrich | Cat#67692-100mL |
| 2-azetidinone CAS: 930-21-2 | Acros | Cat#930212-1g |
| 2-pyrrolidinone CAS: 616-45-5 | TCI | Cat#616455-25g |
| delta-valerolactam CAS: 675-20-7 | TCI | Cat#675207-25g |
| epsilon-caprolactam CAS: 105-60-2 | TCI | Cat#105602-25g |
| phosgene, 15% | Aldrich | Cat#748684-100mL |
| methylamine hydrochloride CAS: 593-51-1 | Aldrich | Cat#593511-100g |
| 1,1-dimethylurea CAS: 598-94-7 | Acros | Cat#598947-5g |
| 1,1,3-trimethylurea CAS: 632-14-4 | Alfa Aesar | Cat#632144-1g |
| 1-methyl-2-imidaolidinone CAS: 694-32-6 | Acros | Cat#694326-500mg |
| 1,3-dimethylurea CAS: 96-31-1 | TCI | Cat#96311-25g |
| ethylene diamine | Roth | Cat#4218.1 |
| FmocNHPEG12COOH | broadpharm | Cat#BP-21632 |
| BG-COOH | Levitz et al. 2017 | N/A |
| BGAG12,400 | this study | N/A |
| 2xBGAG12,400 | this study | N/A |
| BGAG12,400-Cy3 | this study | N/A |
| BGAG12,400-Cy5 | this study | N/A |
| BGAG12,400-Cy3 v2 | this study | N/A |
| BGAG12,400-Cy5 v2 | this study | N/A |
| BGAG12-Cy5 | Acosta-Ruiz et al. 2020 | N/A |
| 2xBGAG12 | Acosta-Ruiz et al. 2020 | N/A |
| BGAG12 | Broichhagen et al. 2015 | N/A |
| 4,4’-diamino azobenzene | Acros | Cat#ACRO401580250 |
| butyryl chloride | Aldrich | Cat#236349-50g |
| ethyl isocyanate | Aldrich | Cat#E33300-5g |
| NaCl | MilliporeSigma™ | Cat#S5886-1KG |
| HEPES | MilliporeSigma™ | Cat#H4034-500G |
| Na+ ascorbate | MilliporeSigma™ | Cat#A7631-100G |
| Thiourea | MilliporeSigma™ | Cat#T8656-100G |
| Na+ pyruvate | MilliporeSigma™ | Cat#P2256-100G |
| CaCl2.2H2O | MilliporeSigma™ | Cat#C3881-500G |
| NMDG | MilliporeSigma™ | Cat#M2004-1KG |
| K-Gluconate | MilliporeSigma™ | Cat#PHR1130-1G |
| phosphocreatine-Na2 | MilliporeSigma™ | Cat#P7936-1G |
| MgATP | MilliporeSigma™ | Cat#A9187-500MG |
| Na2- GTP | MilliporeSigma™ | Cat#G8877-25MG |
| biocytin | MilliporeSigma™ | Cat#B4261-250MG |
| SNAP-Surface Alexa Fluor 546 | New England Biolabs | Cat#S9132S |
| Lipofectamine 2000 | Invitrogen | Cat#11668027 |
| Trypsin | Sigma-Aldrich | Cat# T1763 |
| DMEM/F12 | Sigma-Aldrich | Cat# 6421 |
| DMEM | Gibco | Cat# 41965-039 |
| FBS | Dutscher | Cat# S1900-500 |
| Penicillin-Streptomycin | Gibco | Cat# 15140-122 |
| Trypsin-EDTA | Gibco | Cat# 25300-054 |
| Glutamine | Gibco | Cat# 25030-081 |
| Dexamethasone | Sigma-Aldrich | Cat# D4902 |
| HEPES | Gibco | Cat# 15630-080 |
| Experimental Models: Cell Lines | ||
| HEK 293T Cells | American Type Culture Collection (ATCC) | Cat#CRL-3216; Cat#CR-11268 |
| Experimental Models: Organisms/Strains | ||
| Mouse: STOCK Tg(Grm2-cre)MR90Gsat/Mmucd Mus musculus | Mutant Mouse Resource & Research Center | MMRRC Cat# 034611-UCD |
| Oligonucleotides | ||
| Primer SNAP-mGluR2-mGluR1 Fwd1: TCTTCCACCCTGTAAAGATCCCACACTCCTGCC | This paper | N/A |
| Primer SNAP-mGluR2-mGluR1 Rev1: GCTCCACTCAAGATACTCCTGAGGCAGCTCAAAG | This paper | N/A |
| Primer SNAP-mGluR2-mGluR1 Fwd2: GAGCTGCCTCAGGAGTATCTTGAGTGGAGCAACATC | This paper | N/A |
| Primer SNAP-mGluR2-mGluR1 Rev2: AGGAGTGTGGGATCTTTACAGGGTGGAAGAGCTTTG | This paper | N/A |
| Recombinant DNA | ||
| SNAP-mGluR2 | Doumazane et al., 2011 | N/A |
| GIRK1-F137S | Vivaudou et al., 1997 | N/A |
| SNAP-mGluR2-mGluR5 | Acosta-Ruiz et al. 2020 | N/A |
| SNAP-mGluR2-mGluR1 | This paper | N/A |
| CMV-GCaMP6f | Chen et al., 2013 | Addgene Cat#40755 |
| pCIKv1.1 | Lesage et al., 1992 | N/A |
| Software and Algorithms | ||
| Fiji/ImageJ, v1.8 | Schindelin et al., 2012 | https://imagej.nih.gov/ij/ |
| Prism | GraphPad | https://graphpad.com |
| Clampex | Molecular Devices | https://www.moleculardevices.com |
| Clampfit | Molecular Devices | https://www.moleculardevices.com |
| Origin | Origin Lab Corporation | https://www.originlab.com/, RRID:SCR_002815 |
| Microsoft Excel | Microsoft Office | https://products.office.com/en-us/excel |
| Doric Neuroscience Studio | Doric Lenses | http://doriclenses.com/life-sciences/software/955-doric-neuroscience-studio.html |
| SMART Video Tracking System | Harvard Apparatus | https://www.harvardapparatus.com/smart-video-tracking-system.html |
| SigmaPlot | Systat Software, inc. | https://systatsoftware.com/products/sigmaplot/ |
| Other | ||
| CoolLED pE4000 | CoolLED | https://www.coolled.com/products/pe-4000/ |
| Doric Combined LEDs: 4-LED Model | Doric | LEDC4_385/465/515/625 |