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Schematic of the study design for assessing the influence of different timepoints or gut sections on chicken microbiota. Each sampling day is depicted in orange circles, and the number of birds at different timepoints is also shown. Six chickens were randomly selected for sample collection of the duodenum, cecum, and feces of each bird. The number of gut samples with sufficient sample amount for 16S rRNA sequencing is shown in the dashed box.
