TABLE 1.

Phytochemical composition of NSP01-TE and NSP01 using UHPLC-DAD-ESI-MS/MS analysis.

Peak	tR (min)	Adduct	Calculated m/z	Experimental m/z	Mass accuracy ∆m (mDa)	Experimental MS2 m/z	Identification	Fragment identification	Fragment structure
1	1.3	[M−H]−	315.0711	315.0718	0.7	153.0185	Protocatechuic acid-O-hexoside	Protocatechuic acid
2	4.0	[M−H]−	153.0182	153.0192	1.0	109.0294	Protocatechuic acid	1,2-benzendiol
3	6.7	[M+H]+	243.1492	243.1487	0.5	226.1233	Hup A	Desamino-huperzine A
4	9.7	[M−H]−	179.0339	179.0347	0.8	135.0449	Caffeic acid	3–4,dihydroxystyrene
5	10.3	[M−H]−	-	395.0964	NA	193.0498	Unknown glucuronide	Unknown	Unknown
						175.0395		Unknown	Unknown
						201.0397		Unknown	Unknown
						219.0501		Unknown	Unknown
6	12.0	[M−H]−	353.0867	353.0875	0.8	191.0557	Chlorogenic acida	1,3,4,5-tetrahydroxycyclohexanecarboxylic acid
						161.0249		unamed
						179.0357		Caffeic acid
7	12.6	[M−H]−	-	308.0769	NA	149.0603	Unknown	Unknown	Unknown
						192.0657		Unknown	Unknown
						134.0368		Unknown	Unknown
8	12.8	[M−H]−	-	473.1286	NA	329.0866	Unknown	Unknown	Unknown
						167.0341		Unknown	Unknown
9	14.7	[M−H]−	193.0495	193.0502	0.7	134.0367	Ferulic acid	3–4,dihydroxystyrene (radical)
						178.0264		Caffeic acid (radical)
10	15.0	[M−H]−	-	399.0932	NA	193.0497	Unknown phenolic acid	Unknown	Unknown
						167.0341		Unknown	Unknown
						237.0394		Unknown	Unknown
11	15.7	[M−H]−	-	395.0971	NA	193.0499	Unknown glucuronide	Unknown	Unknown
						219.0504		Unknown	Unknown
						201.0397		Unknown	Unknown
						175.0395		Unknown	Unknown
12	15.9	[M−H]−	-	501.1606	NA	195.0655	Unknown	Unknown	Unknown
						357.1182		Unknown	Unknown
						399.1284		Unknown	Unknown

^aThe intensity of compound 6 in both extracts is very low. However, the fragmentation was possible in NSP01-TE and NSP01 extract and the fragment ions of compound 6 are presented from NSP01-TE extract fragmentation.