FIGURE 4.

Pharmacological blockade of HDAC6 inhibits the activation of the TGF-β signaling pathway and decreases the expression of CTGF and Snail in peritoneal mesothelial cells. (A) Cell lysates were subjected to immunoblot analysis with specific antibodies against TGFβRI, p-Smad3, Smad3, Smad7, and GAPDH. (B) Expression level of TGFβRI was quantified by densitometry and normalized with GAPDH. (C) Expression level of p-Smad3 was quantified by densitometry and normalized with Smad3. (D) Expression level of Smad7 was quantified by densitometry and normalized with GAPDH. (E) Cell lysates were subjected to immunoblot analysis with specific antibodies against CTGF and GAPDH. (F) Expression level of CTGF was quantified by densitometry and normalized with GAPDH. (G) Cell lysates were subjected to immunoblot analysis with specific antibodies against Snail and GAPDH. (H) Expression level of Snail was quantified by densitometry and normalized with GAPDH. Data are represented as the mean ± SEM (n = 3). Means with different superscript letters are significantly different from one another (p < 0.05).