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. 2021 Aug 30;9:724970. doi: 10.3389/fcell.2021.724970

FIGURE 2.

FIGURE 2

HIV Tat-mediated upregulation of CXCR3 in human monocytes. (A) Real-time PCR analysis of CXCR3 mRNA expression in human monocytes exposed to various concentrations of HIV Tat (50, 100, and 200 ng/ml) and heated HIV Tat (50 ng/ml). N = 3. (B) Real-time PCR analysis of CXCR3 mRNA expression in human monocytes exposed to HIV Tat (50 ng/ml) for varying time points. N = 3. (C) Representative Western blot and quantification of CXCR3 in the cell lysates from human monocytes exposed to various concentrations of HIV Tat (50, 100, and 200 ng/ml) and heated HIV Tat (50 ng/ml). N = 4. (D) Flow cytometry analysis of CXCR3 expression on the human monocytes exposed to HIV Tat and heated HIV Tat (50 ng/ml). (E) Representative images of human monocytes exposed to HIV Tat and stained with anti-CXCR3 antibodies. Scale bar 20 μm. N = 3. (F) Quantification of fluorescent intensities of CXCR3 staining in human monocytes (Student’s t-test). One-way ANOVA with post hoc test. All data are presented as mean ± SD of three or four individual experiments (Biological replicates). *p < 0.05 versus control group; **p < 0.01 versus control group; ***p < 0.001 versus control group.