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. 2021 Aug 30;9:724970. doi: 10.3389/fcell.2021.724970

FIGURE 6.

FIGURE 6

Involvement of IRF3 in HIV Tat-mediated upregulation of CXCR3 expression. (A) Representative Western blot and quantification of IRF3 in the nuclear cell lysates of human monocytes transfected with TLR4 siRNA and si-Con followed by HIV Tat exposure. N = 3. (B) Representative Western blot and quantification of p-IRF3, IRF3 in the cell lysates from human monocytes pretreated with TBK1 inhibitor Amlexanox (2 μM), followed by HIV Tat exposure for 15 min. N = 5. (C) Representative Western blot and quantification of IRF3 in the nuclear cell lysates of human monocytes pretreated with TBK1 inhibitor Amlexanox (2 μM), followed by HIV Tat exposure for 15 min. N = 3. (D) Representative Western blot of silencing of IRF3 in human monocytes transfected with IRF3 siRNA (si-IRF3). Representative Western blot and quantification of CXCR3 in the cell lysates from human monocytes transfected with IRF3 siRNA and nonsense siRNA transfected controls (si-Con) followed by HIV Tat exposure. N = 3. One-way ANOVA with post hoc test. All data are presented as mean ± SD of three or four individual experiments (Biological replicates). *p < 0.05 versus control group; **p < 0.01 versus control group; #p < 0.05 versus HIV Tat group.