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. 2021 Aug 30;9:720900. doi: 10.3389/fcell.2021.720900

FIGURE 4.

FIGURE 4

The miR-379/miR-544 cluster KO mice exhibit reduced obesity-associated insulin resistance after feeding with 10 weeks of HFD. (A,B) Blood glucose levels in WT-HFD and KO-HFD mice (n = 4) during a glucose-tolerance test (GTT) (A) and insulin-tolerance test (ITT) (B). *P < 0.05. (C) RT-qPCR analyses of lipogenic genes (Pparγ, Fas, and Scd1) expression in livers from WT-HFD and KO-HFD mice (n = 3). *P < 0.05. (D,E) The protein level of PPARγ in livers from WT-HFD and KO-HFD mice (n = 3) was determined (D) and quantified (E) by Western blot analysis. **P < 0.01. (F,G) Western blot analysis (F) and quantitation (G) of IGF1R, DLK1, phosphorylated AKT (p-AKT), and total AKT in the livers from WT-HFD and KO-HFD mice (n = 3). **P < 0.01.