TABLE 4.
Loss rates of the Ch16 minichromosome in proteasome mutants at permissive (25°C) and semipermissive (30°C) temperatures
| Strain | Background | 25°C
|
30°C
|
||
|---|---|---|---|---|---|
| Chromosome loss per division (%)a | Fold increase in loss rate | Chromosome loss per division (%)a | Fold increase in loss rate | ||
| FY977 | Wild type | 0.07 (4/5,384) | 1 | 0.05 (3/6,579) | 1 |
| 828 | mts2-1 | 0.07 (5/6,950) | 1 | 0.17 (10/6,017) | 3 |
| 831 | mts3-1 | 0.08 (7/9,133) | 1 | 0.27 (17/6,279) | 5 |
| 833 | pad1-1 | 0.09 (3/3,466) | 1 | NDb | NDb |
a
The rate of minichromosome loss was determined by the half-sectoring assay as described previously (3). The total number of colonies examined for each strain is given in parentheses.
b
ND, not determined because the survival of pad1-1 cells was very low at this temperature.