Fig. 2.
Binding of DARPin9.29 fusion proteins to SK-BR-3. (A) mScarlet-DARPin-STII and DARPin-mScarlet-STII plasmid designs, DARPin in orange, mScarlet in red, (GSG)2 in grey, STII in yellow. (B) Schematic representation of DARPin binding to HER2 positive SK-BR-3. (C) Flow cytometry analysis of cells with mScarlet signal for SK-BR-3 and MSC at 37 °C and 20% O2 after 1 h. Error bars showing the range of values from two technical repeats. (D) Confocal microscopy images of SK-BR-3 and MSC cells incubated with DARPin-mScarlet-STII and mScarlet-DARPin-STII. Red = DARPins represented by the red fluorescence of mScarlet; blue = cell nuclei are stained with DAPI (4′,6-diamidino-2-phenylindole). Images were taken at 20× magnification using an Evos Fluorescence Microscope. Scale bars = 200 μm.