Fig. 3.

Design and assembly of the targeted drug delivery system and control samples. Plasmid designs and schematic representation of the protein assembly products. TmEnc-DARPin-STII_miniSOG = encapsulin displaying DARPin loaded with miniSOG; TmEnc-STII = encapsulin only; TmEnc-STII-miniSOG = encapsulin loaded with miniSOG, and miniSOG-STII = miniSOG only. Plasmid component symbols comply with Synthetic Biology Open Language (SBOL) convention. TmEnc (purple) = T. maritima encapsulin gene with His₆ insertion between amino acid 42 and 43; DARPin (orange) = DARPin9.29 gene; STII (yellow) = Strep-tag; miniSOG (blue) = mini Singlet Oxygen Generator; small purple arrow at the 3′end of miniSOG denotes targeted peptide derived from T. maritima ferritin-like cargo protein for recruitment of miniSOG into the capsid; grey = 8 amino acid linker.