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. 2021 Sep 9;6(3):231–241. doi: 10.1016/j.synbio.2021.09.001

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© 2021 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — Specific binding and apoptosis of SK-BR-3 by the DDS (TmEnc-DARPin-STII_miniSOG). (A) Confocal Microscopy image of SK-BR-3 and MSCs after 60-min incubation with DDS showing increased fluorescence intensity correlation to SK-BR-3 cells; Scalebar: 200 μm. It should be noted that SK-BR-3 and MSCs have different morphologies, MSCs are elongated with fibroblastic morphology while the SK-BR-3 have hexagonal shapes and grow in colonies. (B) Flow cytometry analysis showing cell viability percentages from AnnexinV-PI staining after 1 h incubation with the DDS with and without light. Error bars indicate SD across two biological repeats. (C) Percentage apoptotic SK-BR-3 from AnnexinV-PI staining after 1 h incubation in light with control samples (TmEnc-STII_miniSOG, TmEnc-STII and miniSOG-STII). Error bars show SD across triplicate experiments across two biological repeats. T test carried out between * and ** samples returned a P value of 0.031 < 0.05.